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Jackson二抗授权|蛋白|细胞因子

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R&D Systems公司细胞生物学,提供优质的蛋白质,抗体,ELISA试剂盒,实验室试剂,细胞因子.包括Novus Biologicals, BiosPacific, Tocris Bioscience, Boston Biochem和Bionostics. Jackson二抗中国授权代理

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糖基肽酶(N糖苷肽酶F) PNGase F (Peptide N Glycosidase F)  

2017-07-27 11:13:54|  分类: 酶,蛋白质,肽 |  标签: |举报 |字号 订阅

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糖基肽酶(N糖苷肽酶F) PNGase F (Peptide N Glycosidase F)
Enzymes  
 Endoglycosidases and Deglycosylation Kits内切糖苷酶和去糖基化试剂盒   
 Cat. No.  Item 中文名称 Size  英镑? 
 E-PNG01   PNGase F (Peptide N Glycosidase F)  糖基肽酶(N糖苷肽酶F) 0.3 Unit / 60 μl   ?295.00  
 E-rPNG01   Recombinant PNGase F  重组糖基肽酶 0.3 Unit / 60 μl   ?295.00  
 E-PNG05   PNGase F (Peptide N Glycosidase F)  糖基肽酶(N糖苷肽酶F) 1 Unit / 200 μl   ?860.00  
 E-EF01   Endoglycosidase F1  内切糖苷酶 F1 1 Unit / 60 μl   ?309.00  
 E-EF02   Endoglycosidase F2  内切糖苷酶 F2 0.3 Unit / 60 μl   ?309.00  
 E-EF03   Endoglycosidase F3  内切糖苷酶 F3 0.33 Unit / 60 μl   ?309.00  
 E-EH02   Endoglycosidase H  内切糖苷酶H 0.3 Unit / 60 μl   ?309.00  
 E-G001   O-glycosidase  O-糖苷酶 75 mUnit / 60 μl   ?315.00  
 E-XBG01   Endo-Beta-Galactosidase  Endo-β-半乳糖苷酶 0.9 Unit / 60 μl   ?315.00  
 KE-DG01   Enzymatic CarboRelease Kit  酶的糖释放试剂盒  ?447.00  
 KE-DGMX   Enzymatic DeGlycoMx Kit  酶的糖释放试剂盒  ?249.00  
Applications
?Amino acid sequence determination
?X-Ray crystallography
?Removing heterogeneity due to carbohydrates
?Studying carbohydrate ligand binding
?Removing carbohydrate epitopes from antigens
?Studying the role of glycosylation in protein folding and
activity.
Contents
1 vial: PNGase F - 60 μl (0.3 U)
1 vial: 5x Reaction Buffer pH 7 - 400 μl
1 vial: Denaturation Solution - 200 μl
2% SDS/ 1 M β-mercaptoethanol
1 vial: 15% Triton X-100 - 200 μl
QA-Bio? PNGase F cleaves asparagine-linked (N-linked)
oligosaccharides from glycoproteins. PNGase F deaminates
asparagine to aspartic acid, leaving the oligosaccharides
intact.
Denaturation increases the rate of cleavage up to 100x. Most native proteins can still be completely N-deglycosylated but incubation time must be increased. PNGase F will remain active under incubation conditions for at least 72 hours.
PNGase F will not remove oligosaccharides containing Alpha-(1,3)-linked core fucose commonly found on plant glycoproteins; for this purpose, use peptide N-glycosidase A.
Notes                     
* Prices are exclusive of VAT or any other applicable taxes.                    
* The 20μg packs of glycans are supplied as two 10μg vials.                    
*For glycans, we have also provided the nomenclature as assigned by theOxford Notation System (Harvey et al, 2009).                     
   All enzymes listed in this catalog are QABio products. Glycan,O-linked (Cat # SA-CORE1 )                     
  QABio also supply Enzymatic DeGlycoMx Kit (Cat # KE-DGMX) EnzymaticCarboRelease Kit (Cat # KE-DG01)                    
  MAb 4 Glycan Reference Panel (Cat # SA-MAB4) andGalactose-?-(1-3)-GalNAc                     
*Delivery Charges:                     
   ?64 for USA, Far East, Australia   

西宝生物抗原与抗体专业客户团队热心为您服务!请致电手机/微信  17717616967 洽谈糖基化合作
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